|
KMID : 0380020080230010018
|
|
Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 1 p.18 ~ p.22
|
|
|
Exopolysaccharide (EPS) Production by Lactobacillus paracasei KLB58 in Modified Medium under Different Growth Conditions
|
|
So Jae-Seong
|
|
|
Abstract
|
|
|
|
Various probiotic Lactobacillus spp. are known to produce exopolysaccharide (EPS) which has potential health promoting functionality. A Lactobacillus paracasei strain producing EPS was isolated from healthy human. This strain, named L. paracasei KLB58, was grown on modified MRS medium. Experiments were conducted under various growth conditions to optimize the EPS production. Our study showed that incubation temperature played an important role in EPS production. When incubation temperature was changed from to , the increase of EPS production (28.1 mg/ml) was the highest in our experiment. The type of carbon source in the medium also affected EPS production. Galactose was the most effective for EPS production among the carbon sources examined. Using galactose, glucose, lactose and sucrose, the amount of released EPS was 38.9 mg/ml, 35.6 mg/ml, 21.76 mg/ml and 16.9 mg/ml, respectively. However, acidity in growth medium inhibited EPS productivity due to the low growth yield. When grown at pH 4, L. paracasei KLB58 could only produce EPS of 14.6 mg/ml. When the initial amounts of nitrogen and carbon sources were examined, EPS production was not significantly affected by nitrogen source while carbon source affected considerably.
|
|
|
KEYWORD
|
|
|
Exopolysaccharide (EPS), Lactobacillus paracasei KLB 58
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|